Assuntos
Dermatite Esfoliativa/diagnóstico , Dermatomiosite/diagnóstico , Edema/diagnóstico , Dermatoses Faciais/diagnóstico , Glucagonoma/diagnóstico , Neoplasias Pancreáticas/diagnóstico , Dermatite Esfoliativa/etiologia , Dermatomiosite/complicações , Dermatomiosite/imunologia , Diagnóstico Diferencial , Edema/etiologia , Face , Dermatoses Faciais/etiologia , Feminino , Glucagonoma/complicações , Humanos , Pessoa de Meia-Idade , Eritema Migratório Necrolítico/complicações , Eritema Migratório Necrolítico/diagnóstico , Neoplasias Pancreáticas/complicações , Fatores de Transcrição/antagonistas & inibidores , Fatores de Transcrição/imunologiaRESUMO
The 6H1 receptor cloned from activated chicken T cells was initially considered an orphan G-coupled receptor, but was later included in the P2Y family of receptors for purine and pyrimidine nucleotides on the basis of a significant amino acid identity and was renamed P2Y5. Analysis of the expressed sequence tag database revealed the presence of a related sequence exhibiting 63% amino acid identity with this receptor. Starting from this partial sequence, we have isolated a complete clone and identified a 1113 base pair open reading frame encoding a new G-coupled receptor that we have called P2Y5-like. This sequence exhibits 61% identity with the chicken P2Y5 sequence and 30-33% with other P2Y subtypes. A construct encoding this P2Y5-like receptor was transfected into COS-7, 1321N1, and CHO-K1 cells, and expression was documented by Northern blotting. None of the 40 nucleotides and nucleosides tested was able to elicit a response in any of four functional assays: inositol phosphate formation, stimulation or inhibition of cAMP formation, and extracellular acidification measured with a microphysiometer. These results suggest either that the natural ligand of the P2Y5-like receptor is an uncommon nucleotide or alternatively that despite its structural similarity to the P2Y family it is not a nucleotide receptor.
Assuntos
Receptores de Superfície Celular/genética , Receptores Acoplados a Proteínas G , Receptores Purinérgicos P2/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Células COS , Galinhas , Clonagem Molecular , Humanos , Dados de Sequência Molecular , Dobramento de Proteína , Receptores Citoplasmáticos e Nucleares/genética , Alinhamento de Sequência , Transdução de SinaisRESUMO
BACKGROUND: A strong association has been observed between furuncles and Panton-Valentine leukocidin-producing Staphylococcus aureus. CASE REPORTS: Within one year, we cared for three men at the Cayenne hospital who had Staphylococcus aureus septicemia with severe pleuropulmonary involvement originating from furuncular lesions. The Staphylococcus aureus strains isolated from the skin lesions and from blood cultures produced Panton-Valentine leukocidin. CONCLUSION: These cases demonstrate the gravity of S. aureus septicemia in young patients with furunculosis. These cases are the first reported with severe S. aureus infections associated with Panton-Valentine leukocidin producing strains.
